From Lung to Lab: Enhancing Sputum Quality for Rheumatoid Arthritis Research​​

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Presenter Information(s)

Shrostina Magar, Bertina Quach

Abstract or Description

The Studies of Etiology of Rheumatoid Arthritis (SERA) Project is an ongoing research study conducted by the Division of Rheumatology at the Anschutz Medical Campus (AMC). The SERA project aims to identify biomarkers and prevention strategies associated with Rheumatoid Arthritis (RA). RA is a chronic autoimmune disease where the immune system sends antibodies to attack the healthy tissues surrounding the joint. This leads to inflammation, destruction of cartilage, as well as bone damage and deformities.

The SERA project looks at sputum samples, particularly immune cells such as neutrophils and macrophages that can indicate RA related antibodies in the lungs. Participants provide sputum samples through an induced cough procedure using saline mist in a ventilated booth. Sample quality is assessed by measuring cell viability—the percentage of live immune cells—and performing a squamous epithelial cell count, which indicates contamination from saliva.

This study investigates whether specific factors—homogenization speed, recent food intake, and sample transfer method—affect sputum quality. By analyzing changes and contamination levels across these variables, we aim to optimize collection protocols. Improving sample quality is critical to advancing biomarker discovery efforts and ensuring the reliability of lung-based indicators of RA.

Mentor

Kristin Sturm