Sleepy Tea: The Interactions of Apigenin and Chamomile Tea with Glycogen Phosphorylase

Voiceover

Presenter(s)

Taiseer Al-Rajabi

Abstract or Description

The human body’s primary energy source is glucose, and it can be found in abundance in its storage form, glycogen. When the body needs energy from metabolism, glycogen phosphorylase oversees the breakdown of glycogen so glucose can enter the bloodstream. This enzyme can be found in two forms, the active glycogen phosphorylase-a and the less active, folded glycogen phosphorylase-b (GPB). This study aims to observe the effects of chamomile tea, apigenin, and AMP on GPB activity. This was tested by obtaining the kinetic constants of glycogen phosphorylase through running the reverse reaction. Glucose-1-Phosphate was the substrate used, and inorganic phosphate levels were measured to represent GPB activity. Ultimately, high concentrations of both chamomile tea and apigenin caused a decrease in the levels of inorganic pi Using absorbance spectrometry to quantify the rate of product formation.

Using enzyme kinetics to characterize effector behavior

Phosphate, which showed a decreased activity of the enzyme. This was reflected by the Km increasing by 2.44 with chamomile tea, and 7.3 with apigenin. Further tests will be pursued in the future, but it was shown that apigenin can greatly hinder the enzyme’s ability to undergo its reaction.

Mentor

Roberto Nguyen De Guzman