TAGGING IME4 in CANDIDA ALBICANS

Presenter(s)

Hamza Ahmed

Abstract or Description

Candida albicans live as harmless commensals in different part of human body. The overgrowth of these organisms will lead to disease. In addition, a main goal of C. Albicans research is to investigate response of multiple cells to the environment in the human host, in particular to enable colonization and infection. C.albicans is distantly related to Saccharomyces cerevisiae and they share conserved genes at the mating type locus (MTL). However, there are distinctions between the mating cycle of these two species. The C. albicans mating cycle involves concerted chromosome loss instead of meiosis, and they must undergo a phenotypic switch from to white- to opaque in order to mate.In C. albicans, IME4 regulates white-opaque switching, however where the IME4 mutant has more stable opaque cells than wild type (WT). Is the protein expression of IME4- regulated by white-opaque switching? Where is the IME4 protein found in the cell? Using long oligo nucleotide that has a complementary plasmid to the fluorescent protein. about 75bps of IME4, will be inserted in 3’ intergenic region. Once it is generated, it will go under microscopy analysis.