The 2022 CoorsTek Denver Metro Regional Science and Engineering Fair
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Phytoremediation of Lead Contaminated Soil Using Brassica Juncea


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Presenter(s)(s)

Mathangi Kurup

Project Number

JR-EEV-006

Abstract or Description

Heavy metal toxicity has become increasingly concerning for humans, animals, and environments alike. Although there are several remediation methodologies regarding metalloid removal, most are expensive, can be injurious to health, and some even cause secondary pollution. Phytoremediation is a relatively new plant-based approach to remediating hazardous contaminants in the environment using plant species called hyperaccumulators. The objective of this experiment is to prove the efficiency of phytoremediation using the hyperaccumulating plant, brassica juncea (indian mustard), as a method of removing Pb (lead) from soil. Brassica juncea seeds were grown in 4 soil treatments of Pb; 400 ppm (parts per million), 800 ppm, 1200 ppm, and 0 ppm (control). This study was observed for 5 weeks under a controlled environment with light and 35 ml of water twice a day. At the end of the 5 weeks, 5-gram soil samples of Pb-treatments were sent in sandwich bags, to EHS Laboratories VA, for testing. The lab results confirmed that the brassica juncea plant, whilst maintaining good health, had the ability to phytoremediate efficiently and could be used in restoring soil polluted with Pb. The plants had accumulated about 91.08% of Pb in the 400 ppm treatment and 59.6% in the 800 ppm treatment. While 1200 ppm treatment had data irregularities, the results displayed a positive accumulation trend. Overall, the resultant trend clearly indicated that phytoremediation using brassica juncea is effective in remediating Pb-contaminated soil. In conclusion, phytoremediation is an economically viable, sustainable, and environment-friendly solution to remediate metalloid contamination.

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Comments

Daniel Guerrant3 years ago
Excellent experiment. What were the "irregularities" that you saw in the 1200 ppm? It would be interesting to chart how the lead is removed over time, and how much lead does it take to kill these plants. How do your concentrations compare to those found in contaminated (e.g. superfund) sites in the real world?
• • 1 comment
Mathangi Kurup3 years ago
Thank you for your feedback. The irregularities in the 1200 ppm treatment were that it showed an increase of lead concentration in the first data set in comparison to the treatment concentration (1200 ppm) itself. Pot 1 showed 6700 ppm, Pot 2 had 1700 ppm, and Pot 3 had 1700 ppm (please see graph 2 in Results - Graphs) in the initial results (5 weeks) received. However, after sending the soil treatments again after a week, there was a reduction in the lead, thereby still confirming phytoremediation. <br /><br />If I could continue with this experiment, I would be able to get more data and readings of the plant's ability to accumulate lead, including how much it can remove over time and how much it takes to kill the plant, and chart it accordingly.<br /><br />To answer your second question, my lead treatment concentrations were under the toxic limit and were lower than those found in the real world, however, it simulates a toxic environment for the plants. Lead concentrations above 1200 ppm are considered toxic. Superfund sites such as mining and smelting operations have shown lead concentrations higher than 11,000 ppm.<br /><br />Thanks,<br />Mathangi Kurup
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